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C8726

Sigma-Aldrich

Caspase 9 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

Synonym(s):

ICE-Lap6, Mch6

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12352204

Pricing and availability is not currently available.

recombinant

expressed in E. coli (C-terminal histidine-tagged)

Quality Level

assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

>2,000 units/mg protein

mol wt

N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein)
small subunit 13 kDa (subunit containing the histidine tag)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP9(842)

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1 of 4

This Item
SAB5700785SAB5700630SAB3500405
assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

Gene Information

human ... CASP9(842)

Gene Information

human ... CASP9(842)

Gene Information

human ... CASP9(842)

Gene Information

human ... CASP9(842)

specific activity

>2,000 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

recombinant

expressed in E. coli (C-terminal histidine-tagged)

recombinant

-

recombinant

-

recombinant

-

UniProt accession no.

P55211

UniProt accession no.

P55211

UniProt accession no.

P55211

UniProt accession no.

P55211

Biochem/physiol Actions

Activation of caspase-9 (CASP9) through apoptotic stimuli initiates the caspase cascade. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.
When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

Unit Definition

One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.

Physical form

Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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