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L1900

Sigma-Aldrich

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

Synonym(s):

Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.72
Pricing and availability is not currently available.

Quality Level

200

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

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Luria broth base, Miller′s modified Tryptone, yeast extract and sodium chloride

L1900

Luria broth base, Miller′s modified

Luria Agar Base, Miller Tryptone, yeast extract, sodium chloride and agar

L2025

Luria Agar Base, Miller

modified Tryptic Soy Broth- Dehydrated Culture Media NutriSelect® Plus, with bile salts mixture, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC) O157:H7

08069

modified Tryptic Soy Broth- Dehydrated Culture Media

LB Broth (Lennox) Tablet microbial growth medium

L7275

LB Broth (Lennox)

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

form

powder

form

powder

form

powder

form

tablet

application(s)

agriculture
microbiology

application(s)

agriculture
microbiology

application(s)

food and beverages
microbiology
pathogen testing
vitamins, nutraceuticals, and natural products

application(s)

food and beverages
microbiology

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins.[1] Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.[2]

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCL9736
    SLCL6175
    SLCC2309
    SLBX2284
    SLBT0507

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