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M5909

Sigma-Aldrich

IgM Isotype Control from murine myeloma

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

Synonym(s):

Mouse IgM Isotype Control from myeloma

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

MOPC 104E, monoclonal

form

buffered aqueous solution

concentration

200 μg/mL

technique(s)

flow cytometry: suitable

isotype

IgMλ

shipped in

wet ice

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This Item
471348124737798053
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

density

1.076 g/mL at 20 °C

density

1.319 g/mL at 25 °C

density

(1,26 g/cm3 at 20 °C - OECD Test Guideline 109)

density

-

refractive index

n20/D 1.389

refractive index

n20/D 1.416

refractive index

-

refractive index

-

contains

~0.5% phosphoric acid as stabilizer

contains

-

contains

-

contains

-

concentration

~70% in H2O

concentration

70 wt. % in H2O

concentration

-

concentration

-

functional group

hydroxyl

functional group

sulfonic acid

functional group

carboxylic acid, hydroxyl

functional group

carboxylic acid, hydroxyl

General description

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory [1]. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice [2]. IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Application

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM Isotype Control from murine myeloma has been used:
  • in chromatin immunoprecipitation (ChIP) assay[3]
  • LABScreen assays[4]
  • immunofluorescent staining[5]

IgM isotype control antibodies are suitable for use in immunohistochemistry [6][7].
Mouse myeloma proteins tested for use as controls in flow cytometry.

Principle

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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THE ENZYMATIC SYNTHESIS OF FATTY ACIDS BY ALDOL CONDENSATION.
R O Brady
Proceedings of the National Academy of Sciences of the United States of America, 44(10), 993-998 (1958-10-15)
C S Lieber
Biochemical Society transactions, 16(3), 241-247 (1988-06-01)
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Journal of Chromatographic Science, 47.4, 272-278 (2009)
The aldol condensation of acetaldehyde and heptanal on hydrotalcite-type catalysts.
Tichit D, et al.
J. Catal., 219(1), 167-175 (2003)
Acetaldehyde plasma polymer-coated PET fibers for endothelial cell patterning: Chemical, topographical, and biological analysis.
Hadjizadeh, Afra.
Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 94.1, 11-21 (2010)

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Separation of Acetone; Acetic acid; Propionic acid; Ethyl butyrate; Ethanol; Isoamyl acetate; Isobutyric acid; 3-Methyl-2-butanol; Methyl acetate; 1-Propanol; Acetal, ≥98%, FG; 2-Methyl-1-pentanol; Butyl acetate; Ethyl propionate; 3-Pentanol; 2-Pentanol, 98%; Ethyl isobutyrate; Isobutyl acetate; Acetaldehyde; Furfural; Butyric acid; Methanol; Ethyl acetate

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