MCL1

Mammalian Cell Lysis Kit

• Synonym(s): Cell Lysis Reagent
• UNSPSC Code: 12352200
• NACRES: NA.56

Properties

• usage: kit sufficient for 250 extractions (100 mm tissue culture plates)
• Quality Level: 200
• technique(s): immunoprecipitation (IP): suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.

Features and Benefits

• The kit can be used to prepare cell lysis buffer and other modified buffers
• The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Components

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.

Kit Components Also Available Separately

• P8340 Protease inhibitor cocktail 2.5 mL

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 + H332,H315,H318,H335,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable