P0157

Phosphatase, Acid from potato

ammonium sulfate suspension, ≥200 units/mg protein (biuret)

• Synonym(s): (Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
• CAS Number: 9001-77-8
• EC Number: 3.1.3.2 (BRENDA, IUBMB)
• EC Number: 232-630-9
• MDL number: MFCD00131847
• UNSPSC Code: 12352204

Properties

• form: ammonium sulfate suspension
• specific activity: ≥200 units/mg protein (biuret)
• mol wt: 111 kDa
• foreign activity: Apyrase (ATPase) ≤5% of base activity
• storage temp.: 2-8°C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChI key: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Description

General description

Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.

Application

Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.5

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)