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P0157

Sigma-Aldrich

Phosphatase, Acid from potato

ammonium sulfate suspension, ≥200 units/mg protein (biuret)

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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About This Item

CAS Number:
9001-77-8
EC Number:
3.1.3.2 (BRENDA, IUBMB)
EC Number:
232-630-9
MDL number:
MFCD00131847
UNSPSC Code:
12352204

form

ammonium sulfate suspension

specific activity

≥200 units/mg protein (biuret)

mol wt

111 kDa

foreign activity

Apyrase (ATPase) ≤5% of base activity

storage temp.

2-8°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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General description

Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.

Application

Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Physical form

Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.5

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
056K7037
095H7075
081K7055
054K7043
020H7215

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The kinetics and mechanism of the hydrolysis of phosphoric acid esters by potato acid phosphatase.
E F ALVAREZ
Biochimica et biophysica acta, 59, 663-672 (1962-06-04)
Insight into bio-physiological functions of acid phosphatase from potatoes (Solanum tuberosum): A bioanalytical approach.
Rane, S.
Indian Journal of Chemistry, 48a, 15-24 (2009)
H Fujii et al.
Biochimica et biophysica acta, 712(3), 716-718 (1982-09-14)
A method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates was established. Polyprenyl pyrophosphates such as all-trans-octaprenyl and all-trans-nonaprenyl (solanesyl) pyrophosphate can be hydrolyzed completely to the corresponding primary alcohols by the action of potato acid phosphatase in the presence of
Y Mikuni-Takagaki et al.
The Biochemical journal, 268(3), 585-591 (1990-06-15)
In order to understand the mechanism of the post-translational processing of bone phosphoproteins in embryonic bone, periosteal bone strips isolated from 12-day-embryonic-chick tibiae were cultured and the bone proteins labelled with Na2H32PO4. Of the total radiolabelled proteins recovered from the
S I Tu et al.
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
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