P4553
6-Phosphogluconic Dehydrogenase from yeast
lyophilized powder, ≥3.0 units/mg solid
Synonym(s):
6-Phospho-D-gluconate:NADP+ 2-oxidoreductase (decarboxylating), 6-Phosphogluconic Dehydrogenase from yeast
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
yeast
Quality Level
form
lyophilized powder
specific activity
≥3.0 units/mg solid
mol wt
52 kDa
storage temp.
2-8°C
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General description
6-Phosphogluconic Dehydrogenase from yeast comprises of a NADP+ binding domain, α helical hydrophobic interaction domain and a C-terminal domain. It exists as dimer and the C-terminal region is crucial for the substrate binding and release.
Application
6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.
Biochem/physiol Actions
Enzyme activity is determined by measuring the reduction of NAD+ or NADP+. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme.
Unit Definition
One unit will oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO2 per min at pH 7.4 at 37 °C in the presence of NADP+.
inhibitor
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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