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SRP0721

Sigma-Aldrich

AKT1 (E17K), Active

PRECISIO® Kinase, recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

50-75 nmol/min·mg (radiometric)

mol wt

~85 kDa

concentration

0.1 μg/μL

accession no.

NM_005163

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AKT1(207)

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recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

assay

70-95% (SDS-PAGE)

assay

70-95% (SDS-PAGE)

assay

70-95% (SDS-PAGE)

concentration

0.1 μg/μL

concentration

0.1 μg/μL

concentration

0.1 μg/μL

concentration

0.1 μg/μL

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

mol wt

~85 kDa

mol wt

~75 kDa

mol wt

~88 kDa

mol wt

~89-100 kDa

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Application

Useful for kinase assay.

Biochem/physiol Actions

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    T942-3

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