52797-U
HybridSPE®-PL solid phase extraction (SPE) Cartridge
Bed wt. 100 mg, volume 3 mL, pk of 54 ×
Synonym(s):
HybridSPE®-Phospholipid
About This Item
Recommended Products
Product Name
HybridSPE-PL Cartridge, Bed wt. 100 mg, volume 3 mL, pk of 54 ×
material
PE frit (20/40 μm)
polypropylene hardware
Quality Level
description
Cartridge
composition
Bed wt., 100 mg
packaging
pk of 54 ×
technique(s)
solid phase extraction (SPE): suitable
volume
3 mL
matrix active group
zirconia-based phase
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Related Categories
1 of 4
This Item | MSST0017 | MSST0058 | MSST0038 |
---|---|---|---|
recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells |
technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) - | technique(s) mass spectrometry (MS): suitable |
biological source human | biological source human | biological source - | biological source human |
assay ≥98% (SDS-PAGE) | assay 98% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥98% (SDS-PAGE) |
suitability suitable for mass spectrometry (internal calibrator) | suitability suitable for mass spectrometry (standard) | suitability suitable for mass spectrometry (internal calibrator) | suitability suitable for mass spectrometry (internal calibrator) |
mol wt 20812.7 | mol wt - | mol wt - | mol wt - |
General description
The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.
Features and Benefits
- Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
- Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
- 2-3 step generic procedure
- Minimal to no method development
- Available in 96-well and 1 mL cartridge dimensions
Legal Information
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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