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1.50272

Supelco

Purospher®STAR RP-8 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm LiChroCART HPLC cartridge, manuCART holder required for use

Synonym(s):

Purospher® STAR RP-8 endcapped (5 µm) LiChroCART 250-4

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
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Product Name

Purospher® STAR RP-8 endcapped (5 µm), L × I.D. 250 mm x 4 mm LiChroCART HPLC cartridge, manuCART holder required for use

material

stainless steel column

Quality Level

Agency

suitable for USP L7

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

11.2% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

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PROTIAPROTMEM2910
form

dry powder

form

-

form

-

form

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 5 µm LiChroCART 250-4 is a cartridge column which requires a manuCART cartridge column holder part number 1.51486.0001. The manuCART can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART 4-4 part number 1.50250.0001. LiChroCART cartridge columns mounted with a manuCART cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-4 HPLC columns are available part number 1.50037.0001.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 125 bar
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

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F Peter Guengerich et al.
Nature protocols, 4(9), 1245-1251 (2009-08-08)
Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH-P450 reductase in biological preparations. A detailed
Infiltration of pressurized promoter solutions in a mesoporous silica
Punyamurtula and Qiao
Microporous Mater., 103, 5-5 (2007)
Fang Fang et al.
Journal of bacteriology, 191(18), 5743-5757 (2009-07-14)
Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1
Volkan K Köseoğlu et al.
Infection and immunity, 87(7) (2019-04-17)
Shigella flexneri is an intracellular bacterial pathogen that invades epithelial cells in the colonic mucosa, leading to bloody diarrhea. A previous study showed that S. flexneri forms biofilms in the presence of bile salts, through an unknown mechanism. Here, we
Thuc Hue Ly et al.
Nature communications, 7, 10426-10426 (2016-01-28)
Grain boundaries in monolayer transition metal dichalcogenides have unique atomic defect structures and band dispersion relations that depend on the inter-domain misorientation angle. Here, we explore misorientation angle-dependent electrical transport at grain boundaries in monolayer MoS2 by correlating the atomic

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