G1881

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle

Type III, ammonium sulfate suspension

• Synonym(s): GDH/TIM, α-GDH-TPI
• EC Number: 1.1.1.8 (BRENDA, IUBMB)
• MDL number: MFCD00130488
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• type: Type III
• form: ammonium sulfate suspension
• GDH activity: 75-200 units/mg protein (biuret)
• TPI activity: 750-2000 units/mg protein
• storage temp.: 2-8°C

Description

General description

Research area: Neuroscience

α-Glycerophosphate Dehydrogenase-Triosephosphate isomerase (α-GDH-TPI or GDH/TIM) is an enzyme mixture of α-glycerophosphate dehydrogenase (GDH) and triosephosphate isomerase (TPI).

Application

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle has been used in spectrophotometric aldolase assay and fructose 2,6 bisphosphate assay. It has also been used in erythrocyte transketolase (ETK) activity to study the prevalence of and factors associated with thiamin deficiency in obese.

α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca²⁺.

α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate. α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca²⁺. Triosephosphate isomerase (TPI) is an essential enzyme in the glycolytic pathway, responsible for converting dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3 phosphate (GAP). TPI′s role is crucial in enhancing the efficiency of the catabolic process within glycolysis. However, a deficiency in TPI can lead to glycolytic enzymopathies, rare genetic disorders characterized by neurologic dysfunction and hemolytic anemia.

Packaging

(Sold on the basis of α-GDH units.)

Unit Definition

(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

(TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Mixed crystalline suspension in 3.2 M (NH₄)₂SO₄, with 0.05 g/L EDTA, pH 6.0

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable