53702

Pronase^® Protease, Streptomyces griseus

• Synonyme(s) : Pronase^® powder
• CAS Number: 9036-06-0
• Numéro MDL: MFCD00132092
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Description: RTECS - UK9595000
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥45,000 proteolytic units/g dry wt
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Solubilité: water: 10 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.

Note: 1 KU = 1000 units.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Forme physique

Lyophilized from a solution containing calcium acetate as a stabilizer.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Autres remarques

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2