P4860

Protéase from Bacillus licheniformis

≥2.4 U/g

• Synonyme(s) : Protéinase from Bacillus licheniformis, Subtilisine A
• CAS Number: 9014-01-1
• Numéro CE : 3.4.21.62 (BRENDA, IUBMB)
• Numéro CE : 232-752-2
• Numéro MDL: MFCD00132092
• Code UNSPSC : 12352204
• eCl@ss : 32160410
• Nomenclature NACRES : NA.54

Propriétés

• Forme: aqueous solution
• Niveau de qualité: 200
• Activité spécifique: ≥2.4 U/g
• Température de stockage: 2-8°C

Description

Description générale

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.

The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides. It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.

Actions biochimiques/physiologiques

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Remarque sur l'analyse

Activity expressed in Anson Units

Informations légales

A product of Novozyme Corp.

Alcalase is a registered trademark of Novozymes Corp.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H315,H318,H334,H335,H410
• Conseils de prudence: P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310
• Classification des risques: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves