A8097

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

• Synonyme(s) : 3,6-Bis(dimethylamino)acridine hydrochloride
• Formule empirique (notation de Hill) : C₁₇H₁₉N₃ · HCl
• CAS Number: 65-61-2
• Poids moléculaire : 301.81
• Beilstein: 3575587
• Numéro MDL: MFCD00012660
• Code UNSPSC : 12171500
• ID de substance PubChem : 24891317
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Acridine Orange hydrochloride solution, 10 mg/mL in H₂O, ≥95.0% (HPLC)
• Niveau de qualité: 200
• Essai: ≥95.0% (HPLC)
• Forme: solution
• Concentration: 10 mg/mL in H₂O
• Technique(s): microbe id | staining: suitable
• Couleur: orange to red
• εmax: 47-54 at 486-492 nm in water
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
• Clé InChI: VSTHNGLPHBTRMB-UHFFFAOYSA-N

Description

Application

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

Actions biochimiques/physiologiques

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)