I9274

Invertase from baker′s yeast (S. cerevisiae)

200-300 units/mg solid

• Synonyme(s) : β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• CAS Number: 9001-57-4
• Numéro CE : 3.2.1.26 (BRENDA, IUBMB)
• Numéro CE : 232-615-7
• Numéro MDL: MFCD00131410
• Code UNSPSC : 12164500
• Nomenclature NACRES : NA.21

Propriétés

• Niveau de qualité: 100
• Activité spécifique: 200-300 units/mg solid
• Technique(s): photometry: suitable
• Application(s): food and beverages; general analytical
• Activité étrangère: α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%; 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%
• Température de stockage: 2-8°C

Description

Description générale

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Application

Invertase from baker′s yeast (S. cerevisiae) has been used:

• in liquid media (YES) and is used to determine the invertase activity
• supplemented to the reaction mixture in the extraction and analysis of sugars and starch
• for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Actions biochimiques/physiologiques

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Définition de l'unité

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)