C1403

Cholesterol Esterase from Pseudomonas sp.

lyophilized powder, ≥200,000 units/g protein

• Synonyme(s) : Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase
• CAS Number: 9026-00-0
• Numéro CE : 3.1.1.13 (BRENDA, IUBMB)
• Numéro CE : 232-808-6
• Numéro MDL: MFCD00071052
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥200,000 units/g protein
• Poids mol.: ~300 kDa
• Composition: protein, ≥40% biuret
• Température de stockage: −20°C

Description

Application

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Actions biochimiques/physiologiques

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.

Propriétés physiques

Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾⁵M (Linoleate), 6.6 x 10‾⁵M (Oleate)
3.7 x 10‾⁵M (Linolenate), 1.5 x 10‾⁴M (Palmitate)
1.2 x 10‾⁴M (Myristate), 2.3 x 10‾⁵M (Stearate)
Inhibitors: Hg^++, Ag⁺, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)

Définition de l'unité

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)