L7395

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

• Synonyme(s) : 15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
• CAS Number: 9029-60-1
• Numéro CE : 1.13.11.12 (BRENDA, IUBMB)
• Numéro CE : 232-853-1
• Numéro MDL: MFCD00131527
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type I-B
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥50,000 units/mg solid
• Poids mol.: 94 kDa
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Application

Lipoxidase from Glycine max (soybean) has been used:

• to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
• as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
• as a positive control in lipoxygenase inhibitory activity assay

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Actions biochimiques/physiologiques

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.

Définition de l'unité

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Forme physique

Contains stabilizer and NaCl

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)