C2780

Chondroitinase AC from Flavobacterium heparinum

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

• Synonyme(s) : Chondroitin AC lyase
• CAS Number: 9047-57-8
• Numéro CE : 4.2.2.5 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130796
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Conjugué: (Glucosaminoglycan)
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
• Composition: Protein, ~15% Lowry
• Solubilité: 0.02 M phosphate buffer: soluble (pH 7.0)
• Activité étrangère: Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
• Température de stockage: −20°C

Description

Application

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Actions biochimiques/physiologiques

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Autres remarques

Contains potassium phosphate buffer salts and BSA as stabilizer.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Adéquation

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Définition de l'unité

One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

Notes préparatoires

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)