E2039

Chondroitinase AC from Flavobacterium heparinum

recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

• Synonyme(s) : Chondroitin AC lyase
• CAS Number: 9047-57-8
• Numéro CE : 4.2.2.5 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130796
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Conjugué: (Glucosaminoglycan)
• Essai: ≥90% (SDS-PAGE)
• Forme: lyophilized solid
• Activité spécifique: ≥200 units/mg protein
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis.

Définition de l'unité

1 unit is defined as the amount of enzyme that will liberate 1.0 μmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The ε^μΜ for the reaction product Δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Δ-Di-6S (chondroitin sulfate C).

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable