I3019

Interleukin-10 from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

• Synonyme(s) : IL-10
• Numéro MDL: MFCD00212207
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.01-1.50 ng/mL EC₅₀
• Poids mol.: ~18 kDa
• Conditionnement: pkg of 5 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P18893
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Il10(16153)

Description

Application

Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Actions biochimiques/physiologiques

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1⁺ (CD5⁺⁾, and normal B cells. In human, the cellular sources of IL-10 consist of CD4⁺ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion.

Forme physique

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein

Remarque sur l'analyse

The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)