I9276

Interleukin-10 human

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : IL-10
• Numéro MDL: MFCD00131400
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in baculovirus infected Sf21 cells
• Essai: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.1-0.5 ng/mL EC₅₀
• Poids mol.: 18 kDa
• Conditionnement: pkg of 5 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P22301
• Température de stockage: −20°C
• Informations sur le gène: human ... IL10(3586)

Description

Application

Interleukin-10 (IL-10) human has been used to study the effect of IL-10 on cartilage proteoglycan turnover. It has been used as an antigen to analyse the reactivity patterns of autoantibodies in healthy humans and humans suffering from type 1 diabetes mellitus.

Actions biochimiques/physiologiques

IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1⁺ (CD5⁺⁾, and normal B cells. In human, the cellular sources of IL-10 consist of CD4⁺ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion.

Forme physique

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, containing 50 μg bovine serum albumin/μg IL-10

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)