L6632

Lipoxidase from Glycine max (soybean)

Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

• Synonyme(s) : Linoleate:oxygen oxidoreductase, Lipoxygenase
• CAS Number: 9029-60-1
• Numéro CE : 1.13.11.12 (BRENDA, IUBMB)
• Numéro CE : 232-853-1
• Numéro MDL: MFCD00131527
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type V
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: 500,000-1,000,000 units/mg protein
• Poids mol.: 94 kDa
• Température de stockage: 2-8°C

Description

Application

Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Actions biochimiques/physiologiques

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Définition de l'unité

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Forme physique

Suspension in 2.3 M (NH₄)₂SO₄ solution, pH approx. 6.0

Notes préparatoires

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)