M9281

3-Methyladenine

autophagy inhibitor

• Synonyme(s) : 3-MA, 6-Amino-3-methylpurine
• Formule empirique (notation de Hill) : C₆H₇N₅
• CAS Number: 5142-23-4
• Poids moléculaire : 149.15
• Beilstein: 146087
• Numéro CE : 225-908-6
• Numéro MDL: MFCD00010531
• Code UNSPSC : 41106305
• ID de substance PubChem : 24897326
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: synthetic (organic)
• Niveau de qualité: 300
• Essai: ≥99% (HPLC)
• Forme: powder
• Pf: ~300 °C (dec.) (lit.)
• Solubilité: DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow
• Chaîne SMILES: Cn1cnc(N)c2ncnc12
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
• Clé InChI: FSASIHFSFGAIJM-UHFFFAOYSA-N

Description

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

3-Methyladenine has been used as an autophagy inhibitor:

• to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)
• with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells
• to study the interplay between colistin-induced autophagy and apoptosis

Standard for detection of nucleic acid methylation during carcinogenesis.

Actions biochimiques/physiologiques

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).

Notes préparatoires

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable