T7699

Thunderbolt^™ GC10^™ Electrocompetent Cells

for generation of cDNA libraries and DNA plasmid production

• Numéro MDL: MFCD05665590
• Code UNSPSC : 12352200

Propriétés

• Qualité: Molecular Biology
• Forme: suspension
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Thunderbolt GC10 electrocompetent E. coli have a very high transformation efficiency of >1x10¹⁰ cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA. They are comparable to DH10β strain.

Application

Suitable for recovery of high quality plasmid DNA and generation of cDNA libraries from plasmid-based vectors

Caractéristiques et avantages

• Ensures recovery of stable, high quality plasmid DNA as well as methylated DNA
• Renders protection to clonal stocks from T1 and T5 bacteriophage contamination
• Allows for β-galactosidase α-complementation for blue/white screening
• Guaranteed high transformation efficiency
• Convenient 80 μL or 100 μL aliquots

Composants

• Thunderbolt GC10 electrocompetent cells, 5 X 80 μL or 5 X 100 μL (T7074)
• pUC 19 control DNA (10 ng/μL), 10 μL (D2567)

Principe

Thunderbolt GC10 electrocompetent E. coli is K strain bacteria that contain mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5. The host restriction systems are eliminated to allow the cloning of methylated DNA.

Informations légales

GC10 is a trademark of GeneChoice, Inc.

Thunderbolt is a trademark of GeneChoice, Inc.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable