U0880

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

• Synonyme(s) : Urate: oxygen oxoreductase
• CAS Number: 9002-12-4
• Numéro CE : 1.7.3.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00082126
• Code UNSPSC : 12352204
• ID de substance PubChem : 24900619
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥2 units/mg solid
• Poids mol.: 35 kDa by SDS-PAGE
• Température de stockage: −20°C
• Chaîne SMILES: [P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• Clé InChI: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description

Description générale

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.

Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:

• to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
• to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
• for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Actions biochimiques/physiologiques

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Définition de l'unité

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Forme physique

Powder; contains citrate and sucrose

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable