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45124

Sigma-Aldrich

Elastase from porcine pancreas

powder, white, ≥10 U/mg

Synonym(s):

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine pancreas

form

powder

specific activity

≥10 U/mg

mol wt

Mr ~25000

color

white

storage temp.

−20°C

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Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Other Notes

Cleaves polyalanyl-lysine peptides at the alanyl-lysine bond, review; Review, elastase-type proteases
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