D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

• Synonym(s): DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
• CAS Number: 9025-64-3
• EC Number: 3.1.22.1 (BRENDA, IUBMB)
• EC Number: 232-801-8
• MDL number: MFCD00130919
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type IV
• Quality Level: 200
• form: lyophilized powder
• specific activity: 2,000-6,000 Kunitz units/mg protein (biuret)
• mol wt: 45 kDa
• composition: Protein, 60-80%
• foreign activity: RNase ≤0.05%
• storage temp.: −20°C

Description

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

Unit Definition

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Physical form

Contains sodium chloride

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable