N2635

Nucleoside 5′-Diphosphate Kinase from bovine liver

buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)

• Synonym(s): NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
• CAS Number: 9026-51-1
• EC Number: 2.7.4.6 (BRENDA, IUBMB)
• MDL number: MFCD00131734
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• biological source: bovine liver
• Quality Level: 200
• form: buffered aqueous glycerol solution
• specific activity: ≥1,000 units/mg protein (biuret)
• storage condition: (Tightly closed)
• technique(s): activity assay: suitable
• foreign activity: lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signaling

Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.

Application

Nucleoside 5′-Diphosphate Kinase has been used:

• nucleoside diphosphate kinase (NDPK) assays
• coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
• non-metastatic protein 23 (NM23) growth stimulation assay
• in a study to assess inhibition of type I Fc epsilon receptor mediated Ca²⁺ influx and mediator secretion in rat mucosal mast cells
• in a study to investigate protein synthesis in rabbit reticulocytes

Biochem/physiol Actions

Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.

Unit Definition

One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable