P4506

Phosphodiesterase I from Crotalus atrox (Western Diamondback Rattlesnake)

Type IV, crude dried venom

• Synonym(s): 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
• EC Number: 3.1.4.1 (BRENDA, IUBMB)
• EC Number: 232-870-4
• MDL number: MFCD00131883
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type IV
• Quality Level: 200
• form: crude dried venom
• storage temp.: −20°C

Description

General description

Research area: Cell signalingPhosphodiesteraseI (PDEI) exists in three isoforms PDE1A,PDE1B, and PDE1C(2) that are locatedin cardiovascular, central nervous system, and other organs.

Application

Phosphodiesterase I from Crotalus atrox (Western Diamondback Rattlesnake) has been used to analyse its effect on intracellular calcium concentration of neutrophils. It has also been used in combination with other enzymes to determine the activity of phosphatase enzyme.

Phosphodiesterase I, product P4506 from western diamondback rattlesnake, is used in phosphodiesterase activation assays for calmodulin. It is added to hydrolyze AMP.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C. Phosphodiesterase I (PDEI) can hydrolyze 3′,5′-cyclicadenosine monophosphate (cAMP) and 3′,5′-cyclic guanosine monophosphate (cGMP).It forms a complex with Ca²⁺/calmodulin (CaM) that leads to the activationof its catalytic activity.PDEIshows biotherapeutic effects for the treatment of cancer, diseases, neurocognitive,pulmonary, metabolic, heart, renal, neurological disorders, and endocrineabnormalities.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable