CBL419

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

clone BMK13, Chemicon^®, from mouse

• 동의어(들): EMBP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: BMK13, monoclonal
• 종 반응성: human
• 반응하면 안 됨: horse, mouse, rat
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002728.4
• UniProt 수납 번호: P13727
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PRG2(5553)

설명

특이성

CBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.

애플리케이션

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).

Immunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.

Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

물리적 형태

Format: Purified

The monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

저장 및 안정성

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable