T4330

Turbonuclease from Serratia marcescens

recombinant, expressed in E. coli

• 동의어(들): Endonuclease from Serratia marcescens
• CAS Number: 9025-65-4
• EC 번호: 3.1.30.2 (BRENDA, IUBMB)
• MDL number: MFCD00131010
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Serratia marcescens
• Quality Level: 200
• 재조합: expressed in E. coli
• 양식: liquid
• 농도: ≥200,000 units/mL
• 기술: DNA purification: suitable
• 적합성: suitable for cell lysis
• 응용 분야: life science and biopharma
• 저장 온도: −20°C

설명

일반 설명

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

애플리케이션

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.

Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.

Used for the removal of nucleic acid from protein samples.

생화학적/생리학적 작용

Digests native or heat-denatured DNA and RNA.

Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.

Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.

단위 정의

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.

물리적 형태

Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable