10109134001

RNase

from bovine pancreas

• 동의어(들): Rnase
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 100
• 양식: dry powder (prepared from crystallized ribonuclease)
• 특이 활성도: ~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
• 포장: pkg of 500 mg
• 제조업체/상표: Roche
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

특이성

Heat inactivation: 1 mM EDTA

애플리케이션

RNase has been used in DNA ladder assay.

RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

단위 정의

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under the assay conditions; A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.

제조 메모

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash