11426338910

Anti-Fluorescein-AP, Fab fragments

from sheep

• 동의어(들): antibody
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: sheep
• Quality Level: 100
• 결합: alkaline phosphatase conjugate
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: solution
• 포장: pkg of 150 U (200 μl)
• 제조업체/상표: Roche
• 동형: IgG
• 저장 온도: 2-8°C

설명

일반 설명

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

특이성

The polyclonal antibody reacts with free and bound fluorescein.

애플리케이션

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:

• Dot blot
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Southern blot
• Western blot
• Electrochemical biosensing technique for the detection of 16S rRNA

생화학적/생리학적 작용

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

특징 및 장점

Contents
Solution, stabilized

제조 메모

• Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
• ELISA: 150 to 300mU/ml
• Immunohistocytochemistry: 250 to 500mU/ml
• In situ hybridization: 1.5 to 7.5U/ml
• Southern blot: 150mU/ml
• Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available