3HBDB-RO

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

• 동의어(들): 3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH
• EC 번호: 1.1.1.30 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• Quality Level: 100
• 양식: suspension
• 특이 활성도: ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
• 포장: vial of 2 mL (10127833001); vial of 5 mL (10127841001)
• 제조업체/상표: Roche
• 농도: 5 mg/mL
• 불순물: <0.1% LDH; <5% MDH
• 최적 pH: 6.2-6.9(for reduction); 7-9(for oxidation)
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

애플리케이션

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

생화학적/생리학적 작용

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-­hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.

품질

Contaminants: <0.1% LDH, <5% MDH

물리적 형태

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

제조 메모

Stabilizers: NADH, Ca²⁺

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available