H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

• 동의어(들): (R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH
• CAS Number: 9028-38-0
• EC 번호: 1.1.1.30 (BRENDA, IUBMB)
• EC Number: 232-830-6
• MDL number: MFCD00130633
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type V
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: 250-750 units/mg protein
• 정제법: chromatography
• 외래 활성: lactate dehydrogenase ≤0.05%; malate dehydrogenase ≤0.1%
• 저장 온도: −20°C

설명

애플리케이션

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

생화학적/생리학적 작용

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

단위 정의

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

물리적 형태

Lyophilized powder containing Tris buffer salts

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)