H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

lyophilized powder, ≥200 units/mg protein

• 동의어(들): (R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH
• CAS Number: 9028-38-0
• EC 번호: 1.1.1.30 (BRENDA, IUBMB)
• MDL number: MFCD00130633
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Pseudomonas lemoignei)
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥200 units/mg protein
• 정제법: chromatography
• 외래 활성: lactic dehydrogenase ≤0.05%; malic dehydrogenase ≤0.1%
• 저장 온도: −20°C

설명

일반 설명

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

애플리케이션

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

생화학적/생리학적 작용

In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

단위 정의

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

물리적 형태

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)