FORMD-RO

Formate Dehydrogenase

from Candida boidinii

• 동의어(들): FDH, Formate:NAD oxidoreductase
• EC 번호: 1.2.1.2 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: yeast (Candida boidini)
• Quality Level: 100
• 재조합: expressed in E. coli
• 양식: lyophilized
• 특이 활성도: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
• 분자량: 74 kDa
• 포장: pkg of 250 U (10837016001); pkg of 80 U (10244678001)
• 제조업체/상표: Roche
• 농도: ≥10-20 % (w/w)
• 기술: activity assay: suitable
• 색상: off-white
• 최적 pH: 7.5-8.5
• solubility: water: soluble 10 g/L
• 적합성: suitable for gel electrophoresis
• UniProt 수납 번호: O13437
• 응용 분야: life science and biopharma
• 외래 활성: ADH <0.0500%; LDH <0.0500%; MDH <0.1000%
• 저장 온도: 2-8°C

설명

일반 설명

Formate:NAD⁺ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.

특이성

Substrate Specificity and K_m:
Formate dehydrogenase reacts only with formate (K_m = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (K_m 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

애플리케이션

Most widely used in cofactor recycling systems for NADH.

품질

Contaminants: <0.005% LDH and ADH each, <0.1% MDH

단위 정의

One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO₂ in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

저장 및 안정성

Store dry

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash