A4596

ANTI-FLAG^® M1 Agarose Affinity Gel

• 동의어(들): Anti-ddddk, Anti-dykddddk
• UNSPSC 코드: 12352203
• NACRES: NA.32

속성

• 결합: agarose conjugate
• Quality Level: 200
• 항체 생산 유형: primary antibodies
• 양식: suspension
• 동형: IgG12b
• 용량: ≥0.6 mg/mL, gel binding capacity
• 저장 온도: −20°C

설명

일반 설명

ANTI-FLAG^® M1 Agarose Affinity Gel is a purified IgG2B monoclonal antibody covalently attached to agarose.

특이성

Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

애플리케이션

For purification of N-terminal FLAG fusion proteins. Since binding is Ca²⁺-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.

immunoprecipitation (IP): suitable

Elution - FLAG peptide, Glycine, pH 3.5 EDTA

Learn more product details in our FLAG^® application portal.

특징 및 장점

• Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
• Fusion protein may be eluted from affinity resin by mild elution with EDTA.
• A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

물리적 형태

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

기타 정보

ANTI-FLAG^® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable