A5384

Aconitase from porcine heart

• 동의어(들): Aconitate hydratase, Citrate (isocitrate) hydrolyase
• CAS Number: 9024-25-3
• EC 번호: 4.2.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00130335
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Porcine heart
• Quality Level: 200
• 양식: solid
• 분자량: ~66 kDa by gel filtration
• 저장 온도: −20°C

설명

애플리케이션

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

생화학적/생리학적 작용

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

단위 정의

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

분석 메모

Crude preparation containing aconitase activity

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)