I5036

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in E. coli, lyophilized powder, ≥80 units/mg protein

• 동의어(들): IDH1, Isocitrate Dehydrogenase Cytoplasmic
• CAS Number: 9028-48-2
• EC 번호: 1.1.1.42 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 양식: lyophilized powder
• 특이 활성도: ≥80 units/mg protein
• 분자량: 46,658 Da
• 저장 온도: −20°C

설명

일반 설명

Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes. It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence.

생화학적/생리학적 작용

Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH). It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production.

단위 정의

One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 °C (NADP as cofactor).

물리적 형태

Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable