I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

• 동의어(들): threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH
• CAS Number: 9028-48-2
• EC 번호: 1.1.1.42 (BRENDA, IUBMB)
• EC Number: 200-289-5
• MDL number: MFCD00131420
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type IV
• Quality Level: 200
• 양식: buffered aqueous glycerol solution
• 품질: ≤ 0.2% of main activity is obtained with NAD as cofactor.
• 특이 활성도: 3-20 units/mg protein
• 구성: Protein biuret
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

Isocitric dehydrogenase (IDH) has been used:

• for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
• as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
• in isocitrate assay

생화학적/생리학적 작용

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.

단위 정의

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

물리적 형태

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)