C2409

Creatinase from microorganisms

lyophilized powder, ≥4 units/mg solid

• 동의어(들): Creatine Amidinohydrolase
• CAS Number: 37340-58-2
• EC 번호: 3.5.3.3 (BRENDA, IUBMB)
• EC Number: 253-471-1
• MDL number: MFCD00130875
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Actinobacillus spp.)
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥4 units/mg solid
• 분자량: ~100 kDa
• 저장 온도: −20°C

설명

일반 설명

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunits and two defined domains; N and C terminal domains. The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.

애플리케이션

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

생화학적/생리학적 작용

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

물리적 특성

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾²M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu⁺⁺, Hg⁺⁺, Ag⁺
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)

단위 정의

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

물리적 형태

Lyophilized powder containing sugars and EDTA as stabilizers

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)