C9409

Creatinine Deiminase microbial

lyophilized powder, ≥25 units/mg protein

• 동의어(들): Creatinine Deaminase
• CAS Number: 37289-15-9
• EC 번호: 3.5.4.21 (BRENDA, IUBMB)
• EC Number: 253-449-1
• MDL number: MFCD00130878
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥25 units/mg protein
• 분자량: ~260 kDa
• 구성: Protein, ≥15% biuret
• 저장 온도: −20°C

설명

애플리케이션

Creatinine Deiminase microbial has been used:

• to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
• in creating creatinine-sensing membrane for biophysical studies
• to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

생화학적/생리학적 작용

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

물리적 특성

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾³M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag⁺,Hg⁺⁺, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)

단위 정의

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH₃ per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.

물리적 형태

Lyophilized powder containing mannitol as stabilizer

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)