C3172

Creatininase from microorganisms

lyophilized powder, 100-300 units/mg protein

• 동의어(들): Creatinine Amidohydrolase
• CAS Number: 9025-13-2
• EC 번호: 3.5.2.10 (BRENDA, IUBMB)
• EC Number: 232-786-8
• MDL number: MFCD00130877
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: 100-300 units/mg protein
• 분자량: ~175 kDa
• 구성: Protein, 65-85%
• 외래 활성: Creatinase and urease ≤1%; Hexokinase and ATPase ≤0.1%
• 저장 온도: 2-8°C

설명

애플리케이션

Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

생화학적/생리학적 작용

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

물리적 특성

Isoelectric point:4.7
Michaelis constants:3.2 x 10‾²M (Creatinine), 5.7 x 10‾²M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag⁺, Hg⁺⁺, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)

단위 정의

One unit will hydrolyze 1.0 μmole of creatinine to creatine per min at pH 8.0 and 25 °C

물리적 형태

Lyophilized powder containing sucrose and BSA as stabilizers

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)