H2519

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

• 동의어(들): Heparin lyase I, Heparinase
• CAS Number: 9025-39-2
• EC 번호: 4.2.2.7 (BRENDA, IUBMB)
• MDL number: MFCD00131317
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 결합: conjugate (Glucosaminoglycan)
• Quality Level: 200
• 양식: lyophilized solid
• 특이 활성도: ≥200 units/mg protein (enzyme + BSA)
• 분자량: 42.8 kDa
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

애플리케이션

Heparinase I from Flavobacterium heparinum has been used:

• in the digestion of plasma samples
• to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
• in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

생화학적/생리학적 작용

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

단위 정의

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)