H8891

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

• 동의어(들): Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum
• CAS Number: 37290-86-1
• EC 번호: 4.2.2.8 (BRENDA, IUBMB)
• MDL number: MFCD00131319
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Flavobacterium heparinum)
• Quality Level: 200
• 결합: conjugate (Glucosaminoglycan)
• 양식: lyophilized powder
• 특이 활성도: ≥30 units/mg protein (enzyme + BSA)
• 분자량: 70.8 kDa
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

애플리케이션

Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.

생화학적/생리학적 작용

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the H_NY-I and H_NY,6X-G² links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.

단위 정의

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)