N5386

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

• 동의어(들): Endonuclease micrococcal, MNase, Micrococcal endonuclease
• CAS Number: 9013-53-0
• EC 번호: 3.1.31.1 (BRENDA, IUBMB)
• EC Number: 232-748-0
• MDL number: MFCD00131731
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Staphylococcus aureus
• Quality Level: 200
• 양식: powder
• 특이 활성도: 100-300 units/mg protein
• 구성: Protein, ≥40% E1%/280 (Balance primarily sodium acetate)
• 기술: DNA extraction: suitable; DNA purification: suitable
• 적합성: suitable for molecular biology
• 응용 분야: cell analysis
• 저장 온도: −20°C
• 유전자 정보: Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

설명

애플리케이션

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

생화학적/생리학적 작용

Hydrolyzes 5′-phosphodiester bonds of DNA.

기타 정보

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)