S8633

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

• 동의어(들): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS Number: 9031-54-3
• EC 번호: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 양식: buffered aqueous glycerol solution
• Quality Level: 200
• 특이 활성도: 100-300 units/mg protein (Lowry)
• 저장 온도: 2-8°C

설명

애플리케이션

Sphingomyelinase from Staphylococcus aureus has been used to:

• induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
• determine the concentration of sphingomyelin from serum samples
• enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

생화학적/생리학적 작용

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

단위 정의

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

물리적 형태

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)