S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

• 동의어(들): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS Number: 9031-54-3
• EC 번호: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: buffered aqueous glycerol solution
• Quality Level: 200
• 특이 활성도: ≥100 units/mg protein (Lowry)
• 분자량: 34.2 kDa
• 외래 활성: Phospholipase C ≤1%
• 저장 온도: 2-8°C
• 유전자 정보: Bacillus cereus ATCC 10987 ... spH(2751530)

설명

일반 설명

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

애플리케이션

Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
• in lipoprotein modification for the generation of cholesterol crystals in vitro
• to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

생화학적/생리학적 작용

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

단위 정의

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

물리적 형태

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable