890700P

12:0 EPC (Cl Salt)

Avanti Research^™ - A Croda Brand 890700P, powder

• Synonym(s): 1,2-dilauroyl-sn-glycero-3-ethylphosphocholine (chloride salt)
• Empirical Formula (Hill Notation): C₃₄H₆₉NO₈PCl
• CAS Number: 474945-22-7
• Molecular Weight: 686.34
• MDL number: MFCD22495356
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• form: powder
• packaging: pkg of 1 × 10 mg (890700P-10mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand 890700P
• lipid type: cationic lipids; transfection
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: O=P(OCC[N+](C)(C)C)(OC[C@]([H])(OC(CCCCCCCCCCC)=O)COC(CCCCCCCCCCC)=O)OCC.[Cl-]
• InChI: 1S/C34H69NO8P.ClH/c1-7-10-12-14-16-18-20-22-24-26-33(36)39-30-32(31-42-44(38,40-9-3)41-29-28-35(4,5)6)43-34(37)27-25-23-21-19-17-15-13-11-8-2;/h32H,7-31H2,1-6H3;1H/q+1;/p-1/t32-,44?;/m1./s1
• InChI key: QWVQEMZYQQUQCH-XFSYIJCRSA-M

Description

General description

1,2-dilauroyl-sn-glycero-3-ethylphosphocholine (12:0 EPC) is a cationic phospholipid. 12:0 EPC is a short-chain analog of dioleoyl ethylphosphatidylcholine. O-alkyl phosphatidylcholines constitute the first chemically stable triesters of biological lipid structures and the first cationic derivatives of phospholipids consisting entirely of biological metabolites linked with ester bonds. The lipid has low toxicity and is biodegradable.

Application

1,2-dilauroyl-sn-glycero-3-ethylphosphocholine (12:0 EPC (Cl Salt)) is suitable for use in lipoplex preparation and to study the lipoplex transfection activity. It might also be used in lipid mixtures to study its cubic phases of formation, stability and phase transitions.

Biochem/physiol Actions

1,2-dilauroyl-sn-glycero-3-ethylphosphocholine (12:0 EPC) is preferred for transfection of cultured primary endothelial cells. 12:0 EPC forms lamellar lipoplexes possessing high to superior transfection activity. Chemically synthesized cationic lipids are applicable as non-viral gene carriers. Variation in the hydrocarbon chain of phosphatidylcholine (PC) derivatives affects transfection efficiency.

Packaging

5 mL Clear Glass Sealed Ampule (890700P-10mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3