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03-0153-00

Millipore

SMC® Glucagon High Sensitivity Kit

1 kit sufficient for 96 wells, input: plasma

Synonym(s):

Glucagon Single Molecule Counting Kit, Glucagon ultra-sensitive immunoassay, Pro-glucagon immunoassay kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Pricing and availability is not currently available.

usage

 kit sufficient for 96 wells

species reactivity

rat, human, mouse

manufacturer/tradename

SMC®

assay range

sensitivity: 0.78 pg/mL
(lower limit of quantification)

inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.20-200 pg/mL

technique(s)

ELISA: suitable
single molecule counting: suitable

input

plasma

compatibility

for use with SMCxPRO® and Erenna (validated)

shipped in

wet ice

storage temp.

2-8°C

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SRP0643SRP0641SRP0635
recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

mol wt

54 kDa

mol wt

54 kDa

mol wt

54 kDa

mol wt

54 kDa

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

Gene Information

human ... CD274(29126)

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

The SMC® Glucagon High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.

Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2)The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Specificity

Glucagon (human) was found to be 100% detectable.
Oxyntomodulin (human) was found to be <0.5% detectable.
GLP-1 (human) was not found to be detectable.
GLP-2 (human) was not found to be detectable.
GIP (human) was not found to be detectable.

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jennifer L Warner-Schmidt et al.
Biological psychiatry, 68(6), 528-535 (2010-07-02)
The protein p11 (also called S100A10) is downregulated in human and rodent depressive-like states. Considerable experimental evidence also implicates p11 in the mechanism of action of antidepressant drugs and electroconvulsive seizures, in part due to its interaction with specific serotonin
Per Svenningsson et al.
Nature reviews. Neuroscience, 14(10), 673-680 (2013-09-05)
Studies of the multifunctional protein p11 (also known as S100A10) are shedding light on the molecular and cellular mechanisms underlying depression. Here, we review data implicating p11 in both the amplification of serotonergic signalling and the regulation of gene transcription.
p11 and its role in depression and therapeutic responses to antidepressants
Svenningsson P, et al.
Nature Reviews. Neuroscience, 14(10), 673-680 (2013)
Disruption of annexin II/p11 interaction suppresses leukemia cell binding, homing and engraftment, and sensitizes the leukemia cells to chemotherapy
Gopalakrishnapillai A, et al.
Testing, 10(10), e0140564-e0140564 (2015)
Jiqiang Guo et al.
Scientific reports, 6, 17029-17029 (2016-01-30)
Depression is one of the major side effects of interferon alpha (IFN-α) treatment, but the molecular mechanism underlying IFN-α-induced depression remains unclear. Several studies have shown that the serotonin receptors 5-HTR1b and 5-HTR4 play key roles in the anti-depression effects

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